The strike is aimed at pressurising President Joseph Kabila to back a political deal which fell apart week.
The deal outlined the creation of a new government paving the way for a general election.
Mr Kabila was supposed to step down last year but the vote to replace him was not held.
Security forces have been deployed to protect business owners who have chosen to ignore the strike.
Mr Kabila’s second and final term came to an end last November but he failed to relinquish power, with his government saying elections could not be organised on schedule because of financial and logistical difficulties.